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Focused ultrasound was produced with a 12-cm diameter piezoelectric ceramic transducer. The system was operated using one of the several therapeutic transducers with the focal length of 90 to 160 mm. For each focal length, there is a choice of two transducers depending on the target depth: one operates at 0.8 MHz with 135-mm focal length and the other operates at 1.6 MHz with 90-mm focal length. The choice of transducers depends on the depth of target lesion, with the most commonly used parameters in this study were 0.8-MHz operating frequency and 135-mm focal length. In the centre of the transducer, there is a 3.5- to 5.0-MHz diagnostic ultrasound (US) imaging probe which is used as the real-time imaging unit of the system, guiding the target tissue volume, monitoring the energy deposition and the therapeutic effect, and also controlling the US exposure based on the feedback digital data from the ultrasonograms in the process of HIFU treatment. The therapeutic transducer and diagnostic imaging device were integrated into one transducer, and their beams were completely overlaid each other in the longitudinal diretion. The integrated transducer is moved by electric motors and can be moved smoothly in six directions, including three orthogonal directions (x, y, z), rotation along the ultrasound beam axis (θ), and rotation along the long or short axis of the bed (γ, φ) . Through computer control, the imaging probe was placed either against the skin or at a distance from the skin in water for pre-treatment imaging. The integrated transducer was mounted in a degassed water reservoir with the ultrasound beam directed upward. The ultrasound beam of the therapeutic transducer and the imaging probe overlapped completely, so that the longitudinal axis of the high-intensity focused ultrasound beam is in the two-dimensional US imaging plane. A calibrated polyvinylidene difluoride membrane hydrophone with a spot diameter of 0.5 mm was used to map the acoustic pressure field of the focused transducer at focal peak intensities of 200~300 W/cm2 [2]. The focal region was cigar shaped, with dimensions of 9.8 mm along the beam axis and 1.3 mm in the transverse direction. The absorbing target method was used to measure the total acoustic power output in degassed water at 21°C [6, 14].

Focused ultrasound was produced with a 12-cm diameter piezoelectric ceramic transducer. The system was operated using one of the several therapeutic transducers with the focal length of 90 to 160 mm. For each focal length, there is a choice of two transducers depending on the target depth: one operates at 0.8 MHz with 135-mm focal length and the other operates at 1.6 MHz with 90-mm focal length. The choice of transducers depends on the depth of target lesion, with the most commonly used parameters in this study were 0.8-MHz operating frequency and 135-mm focal length. In the centre of the transducer, there is a 3.5- to 5.0-MHz diagnostic ultrasound (US) imaging probe which is used as the real-time imaging unit of the system, guiding the target tissue volume, monitoring the energy deposition and the therapeutic effect, and also controlling the US exposure based on the feedback digital data from the ultrasonograms in the process of HIFU treatment. The therapeutic transducer and diagnostic imaging device were integrated into one transducer, and their beams were completely overlaid each other in the longitudinal diretion. The integrated transducer is moved by electric motors and can be moved smoothly in six directions, including three orthogonal directions (x, y, z), rotation along the ultrasound beam axis (θ), and rotation along the long or short axis of the bed (γ, φ) . Through computer control, the imaging probe was placed either against the skin or at a distance from the skin in water for pre-treatment imaging. The integrated transducer was mounted in a degassed water reservoir with the ultrasound beam directed upward. The ultrasound beam of the therapeutic transducer and the imaging probe overlapped completely, so that the longitudinal axis of the high-intensity focused ultrasound beam is in the two-dimensional US imaging plane. A calibrated polyvinylidene difluoride membrane hydrophone with a spot diameter of 0.5 mm was used to map the acoustic pressure field of the focused transducer at focal peak intensities of 200~300 W/cm2 [2]. The focal region was cigar shaped, with dimensions of 9.8 mm along the beam axis and 1.3 mm in the transverse direction. The absorbing target method was used to measure the total acoustic power output in degassed water at 21°C [6, 14]..

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Triple-negative breast cancers (TNBC) lack expression of three common cell surface receptors, i.e., estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor-2 (HER2). Accordingly, TNBCs are associated with fewer treatment options and a relatively poor prognosis. Having screened a National Cancer Institute natural compound library, the purpose of this study was to investigate the bioactivity of compound C4 (Crassin) in TNBC cells.. Expression and localization of recombinant hIL-18-EGFP

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